个人简介

    何灵娟（1986-），河北承德人。2010年毕业于南京农业大学，获学士学位。2010-2015年在中国科学院营养科学研究所攻读博士学位，从事心脏发育与再生相关研究。2015-2017年在中国科学院生化细胞所进行博士后研究，主要从事基于Cre-loxP和Dre-rox双同源重组酶系统的遗传谱系示踪新技术的开发和应用，以及器官发育与再生过程中细胞起源及命运的相关研究。2017-2020年在中科院生化细胞所任副研究员。何灵娟博士于2021年初全职加入西湖大学任研究员。曾获得国家优秀青年科学基金、上海市青年科技启明星等。

学术成果

      遗传谱系示踪技术是揭示体内特定类型细胞在器官发育、组织稳态、修复再生和疾病过程中细胞增殖、迁移、分化或去分化等现象的有效研究方法。传统的遗传谱系示踪技术主要基于Cre-loxP同源重组酶系统，该技术广泛的应用于发育、再生和干细胞等领域。何灵娟博士开发了基于Cre-loxP和Dre-rox的双同源重组酶系统的遗传谱系示踪新技术，可以实现更为精准的体内细胞命运示踪，并且极大地拓宽了示踪技术的使用范围。她利用该技术系统的探索了心脏发育与再生过程中冠状血管的起源和形成机制以及心脏损伤后心肌细胞的来源。此外，何灵娟博士近期又开发了基于双同源重组酶系统的细胞增殖检测新技术——ProTracer，不同于传统的组织化学染色方法，该新技术引入了遗传学技术将增殖的细胞进行遗传标记，实现了三个方面的突破：长时程不间断检测细胞增殖、检测特定细胞谱系的增殖、以及活体检测细胞增殖。ProTracer可以广泛的应用到多种组织器官发育再生等过程中细胞增殖相关研究，何灵娟博士首先利用ProTracer研究了肝脏在生理稳态和损伤再生过程中新生肝细胞的来源，为肝脏疾病的病理生理过程和临床治疗研究奠定了重要的理论基础。

本课题组未来的研究方向主要包括：

（1）遗传谱系示踪新技术的开发及应用

（2）肝脏、肾脏、皮肤等多种器官再生修复中细胞的起源和命运机制研究

（3）器官再生的细胞及分子机制研究

代表论文

1. He S, Guo Z, Zhou M, Wang H, Zhang Z, Shi M, Li X, Yang X, He L*. Spatial-temporal proliferation of hepatocytes during pregnancy revealed by genetic lineage tracing. Cell Stem Cell. 2023, S1934-5909(23)00323.

2. Liu X, Weng W, He L*, Zhou B*. Genetic recording of in vivo cell proliferation by ProTracer. Nat Protoc. 2023, 18(7):2349-2373.  (*Co-corresponding author)

3. He L^#, Pu W^#, Liu X^#, Zhang Z, Han M, Li Y, Huang X, Han X, Li Y, Liu K, Shi M, Lai L, Sun R, Wang Q, Ji Y, Tchorz J, Zhou B. Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair. Science 2021, 371(6532):eabc4346. (^#Co-first author)

Comments in：

(1) “In the zone for liver proliferation”, Science, 2021

(2) “Novel insights into liver homeostasis and regeneration”, Nat Rev Gastroenterol Hepatol, 2021

4. Han X^#, Zhang Z^#, He L^#, Zhu H^#, Li Y, Pu W, Han M, Zhao H, Liu K, Li Y, Huang X, Zhang M, Jin H, Lv Z, Tang J, Wang J, Sun R, Fei J, Tian X, Duan S, Wang Q, Wang L, He B, Zhou B. A suite of new Dre-recombinase drivers markedly expands the ability to perform intersection genetic targeting. Cell Stem Cell 2021,28(6):1160-1176.  (^#Co-first author)

Comment in：

“Sweet Dre-ams about intersectional genetic”, Cell Stem Cell, 2021

5. Liu X^#, Pu W^#, He L^#, Li Y, Zhao H, Li Y, Liu K, Huang X, Weng W, Wang QD, Shen L, Zhong T, Sun K, Ardehali R, He B, Zhou B. Cell proliferation fate mapping reveals regional cardiomyocyte cell-cycle activity in subendocardial muscle of left ventricle. Nat Commun. 2021;12:5784. (^#Co-first author)

6. He L, Nguyen N, Ardehali R, Zhou B. Heart Regeneration by Endogenous Stem Cells and Cardiomyocyte Proliferation: Controversy, Fallacy, and Progress. Circulation. 2020,142(3):275-291.

7. He L*, Han M, Zhang Z, Li Y, Huang X, Liu X, Pu W, Zhao H, Wang QD, Nie Y, Zhou B. Re-assessment of c-Kit⁺ Cells for Cardiomyocyte Contribution in Adult Heart. Circulation. 2019,140(2):164-166. (*Co-corresponding author)

8. He L, Zhou B. The Formation of Coronary Vessels in Cardiac Development and Disease. Cold Spring Harb Perspect Biol. 2019, doi:10.1101/cshperspect.a037168.

9. Li Y^#, He L^#, Huang X, Bhaloo SI, Zhao H, Zhang S, Pu W, Tian X, Li Y, Liu Q, Yu W, Zhang L, Liu X, Liu K, Tang J, Zhang H, Cai D, Ralf AH, Xu Q, Lui KO, Zhou B. Genetic Lineage Tracing of Nonmyocyte Population by Dual Recombinases. Circulation. 2018, 138(8): 793-805. (^#Co-first author)

Comment in:

 “Undeniable Evidence That the Adult Mammalian Heart Lacks an Endogenous Regenerative Stem Cell”, Circulation, 2018

10. He L*, Li Y, Huang X, Li Y, Pu W, Tian X, Cai D, Huang H, Lui KO, Zhou B. Genetic lineage tracing of resident stem cells by DeaLT. Nat Protoc. 2018, 13(10): 2217-2246. (Cover story) (*Co-corresponding author)

11. He L, Li Y, Li Y, Pu W, Huang X, Tian X, Wang Y, Zhang H, Liu Q, Zhang L, Zhao H, Tang J, Ji H, Cai D, Han Z, Han Z, Nie Y, Hu S, Wang QD, Sun R, Fei J, Wang F, Chen T, Yan Y, Huang H, Pu WT, Zhou B. Enhancing the precision of genetic lineage tracing using dual recombinases. Nat Med. 2017, 23(12):1488-1498.

12. He L, Huang X, Kanisicak O, Li Y, Wang Y, Li Y, Pu W, Liu Q, Zhang H, Tian X, Zhao H, Liu X, Zhang S, Nie Y, Hu S, Miao X, Wang QD, Wang F, Chen T, Xu Q, Lui KO, Molkentin JD, Zhou B. Preexisting endothelial cells mediate cardiac neovascularization after injury. J Clin Invest. 2017, 127(8):2968-2981.

Comments in:

(1) “Where do new endothelial cells come from in the injured heart?”, Nat Rev Cardiol, 2017

(2)  and “The relationship between cardiac endothelium and fibroblasts: it’s complicated”, J Clin Invest, 2017

13. He L, Zhou B. Cardiomyocyte proliferation: remove brakes and push accelerators. Cell Res. 2017, 27(8):959-960. (Research Comment)

14. He L, Liu Q, Hu T, Huang X, Zhang H, Tian X, Yan Y, Wang L, Huang Y, Miquerol L, Wythe JD, Zhou B. Genetic lineage tracing discloses arteriogenesis as the main mechanism for collateral growth in the mouse heart. Cardiovasc Res. 2016, 109(3): 419-430.

15. Tian X^#, Hu T^#, Zhang H^#, He L^#, Huang X, Liu Q, Yu W, He L, Yang Z, Yan Y, Yang X, Zhong TP, Pu WT and Zhou B. Vessel formation. De novo formation of a distinct coronary vascular population in neonatal heart. Science 2014, 345:90-94. (^#Co-first author)

Comment in:

 “A crowning achievement for deciphering coronary origins.”, Science, 2014

联系方式

    电子邮箱：helingjuan@westlake.edu.cn

本课题组2021年开始招收博士研究生，同时有多个科研助理、博士后岗位虚位以待，期待有志者加入！

请将个人简历及相关附件证明材料以一个pdf文件形式发送到helingjuan@westlake.edu.cn,邮件标题及附件简历命名方式为：应聘岗位-本人姓名。