个人简介

　　宋春青，河北石家庄人，2014年于北京生命科学研究所/中国农业大学获得博士学位，博士期间的研究方向为线粒体蛋白组学和生物体衰老的机制研究。2015年3月至2019年9月在麻省大学医学院RNA治疗研究所进行博士后研究，研究肝癌发生的分子机制和利用基因编辑技术纠正基因突变相关的遗传病。2019年10月全职加入西湖大学任特聘研究员。

学术成果和未来研究方向

实验室主要致力于研究肝脏相关疾病（即肝癌和组织再生）的机制，改进CRISPR相关策略（包括先导编辑）用于遗传病校正（包括1型遗传性酪氨酸血症，HT1和α1-抗胰蛋白酶缺乏症， AATD)和活细胞DNA成像。

我们开发了一种CRISPR FISHer实时成像系统，通过相分离介导的信号放大（https://www.nature.com/articles/s41422-022-00712-z），可以对活细胞中的非重复DNA进行高灵敏度成像。

此外，宋博士通过全基因组CRISPR筛选确定了MAPK调节因子是肝脏中至关重要的肿瘤抑制因子；宋博士帮助提高了对CRISPR工程的理解：(1)化学修饰的向导RNA改进了基因组编辑，减少了体内外脱靶活性；(2)腺嘌呤碱基编辑（ABE）应用于成年小鼠酪氨酸血症模型。

专利

Structure-guided chemical modification of guide RNA and its applications. US16/256,003, PCT/US2019/014865.

CRISPR-based imaging system and use thereof, PCT/CN2023/088712.

电子转运裂解质谱预处理与鉴定方法 CN201010515241

代表论文   

1. Huang, X. Y.^#, Yang, D.^#, Liu, Y.^# et al., Shi, J., & Song, CQ. SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes. Cell Reports, 2025, 44(6).

2. Li, Z.^#, Li, Z.^#, Zhang, Y., et al, Zhang, Z., Song, CQ^＊, Wu, J.^＊, & Shen, E. Z.^＊ (2024). Mammalian PIWI–piRNA–target complexes reveal features for broad and efficient target silencing. Nature Structural and Molecular Biology, 2024, 31(8), 1222–1231.

3. Lyu, X. Y., Deng, Y., Huang, X. Y., Li, Z. Z., Fang, G. Q., Yang, D., Wang, F. L., Kang, W., Shen, E. Z.^＊,&  Song CQ^＊. CRISPR FISHer enables high-sensitivity imaging of nonrepetitive DNA in living cells through phase separation-mediated signal amplification. Cell Research, 2022, 32(11), 969–981.

4. Yang, D., & Song, CQ. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs). In Methods in Molecular Biology, 2023, Vol. 2606, 159–170

5. Yin, C. Q., & Song, CQ. Tumor Intrinsic Immunogenicity Suppressor SETDB1 Worsens the Prognosis of Patients with Hepatocellular Carcinoma. Cells, 2024,13(24).

6. Fu, X. W., &  Song CQ. Identification and Validation of Pyroptosis-Related Gene Signature to Predict Prognosis and Reveal Immune Infiltration in Hepatocellular Carcinoma. Frontiers in Cell and Developmental Biology, 2021,9.

7. Wu S^#, Li Q^#, Yin C, Xue W^＊, Song CQ^＊. Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases. Theranostics, 2020 10:4374-4382

8. Song CQ^#, Jiang T^#, et al. Adenine base editing in an adult mouse model of tyrosinemia. Nature Biomedical Engineering. 2020. 4:125–130

9. Yin H^#, Song CQ^#, et al. Partial DNA-guided Cas9 enables genome editing with reduced off-target activity. Nature Chemical Biology. 2018;14(3):311-316.              

10. Song CQ^#, Li Y^#, et al. Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice. Gastroenterology. 2017;152(5):1161-1173.e1.

11. Song CQ, et al. In vivo genome editing partially restores alpha1-antitrypsin in a murine model of AAT deficiency. Human Gene Therapy. 2018;29(8):853-860. F1000

12. Song CQ, Xue W. CRISPR-Cas-related technologies in basic and translational liver research. Nature Reviews Gastroenterology & Hepatology. 2018;15(5):251-252.

13. Yin H, Song CQ, et al. Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing. Nature Biotechnology. 2017;35(12):1179-1187.

14. Yin H, Song CQ, et al. Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo. Nature Biotechnology. 2016;34(3):328-33.

15. Shen EZ^#, Song CQ^#, Lin Y^#, et al. Mitoflash frequency in early adulthood predicts lifespan in Caenorhabditis elegans. Nature. 2014;508(7494):128-32.

16. Liu C^#, Song CQ^#, et al. pQuant improves quantitation by keeping out interfering signals and evaluating the accuracy of calculated ratios. Analytical Chemistry. 2014;86(11):5286-94.

All the publications can be accessed through Google Scholar:

https://scholar.google.com/citations?user=SYrvfr0AAAAJ&hl=en

联系方式

电子邮箱： songchunqing@westlake.edu.cn

本实验室长期招聘基因编辑技术相关方向的博士后，欢迎有志人士联系！