新闻与活动 活动信息

生命科学专题学术讲座 | Haifeng Wang: CRISPR tools for studying the three-dimensional genome in living cells

时间

2022年8月12日
10:00-11:30

地点

云谷校区E9-109

主持

西湖大学生命科学学院 讲席教授 于洪涛

受众

全体师生

分类

学术与研究

生命科学专题学术讲座 | Haifeng Wang: CRISPR tools for studying the three-dimensional genome in living cells

    时间:8月12日星期五10:00-11:30    

Time:10:00-11:30 AM,Fri., Aug. 12,2022

主持人:西湖大学生命科学学院讲席教授 于洪涛

    Host:Dr. Hongtao Yu, Chair professor, School of Life Sciences

    地点:西湖大学云谷校区E9-109

    Venue:Room E9-109, Yungu Campus

主讲嘉宾/Speaker:


Dr. Haifeng Wang, School of Life Sciences, Tsinghua University


Haifeng Wang carried out her Ph.D work in biochemistry, molecular, cellular and developmental biology at University of California, Davis, CA, where she studied the mechanism of anaphase B chromosome segregation during mitosis, focusing on the cooperation between mitotic motors and microtubule dynamics. After that, Wang completed postdoctoral research in Department of Bioengineering at Stanford University, where she developed CRISPR-based tools to study spatiotemporal genome organization, including the CRISPR-Genome organization (GO) platform for programmable control of spatial genome organization (Cell, 2018) and the CRISPR LiveFISH platform for DNA and RNA imaging in living cells (Science, 2019). She also wrote reviews on CRISPR technologies (Annu. Rev. Biochem.,2016) and its applications in 3D genome research (Nat. Rev. Genet.,2021).

Wang moved to School of Life Sciences, Tsinghua University as an assistant professor in 2021. She also works as an independent PI in Tsinghua University-Peking University Joint Center for Life Sciences. She is enthusiastic about applying bioengineering tools to investigate complex biological mechanisms. In the post-genomic era, elucidating the function and control of 3D chromatin structure and organization, and its relationship with various nuclear compartments has been a central goal in genomics and cell biology. Building on her experiences in cell biology and bioengineering, Wang is particularly interested in developing and applying CRISPR toolkits to study the function and control of 3D genome organization and transcriptomedynamics during development and disease.


讲座摘要/Abstract:

The CRISPR techniques provide powerful toolkits for us to probe 3D genome structure, function and dynamics. We developed the CRISPR-Genome Organization (CRISPR-GO) platform for programmable control of spatial genome organization, and the CRISPR live-cell fluorescence in situ hybridization (LiveFISH) platform for DNA and RNA live-cell imaging.

The CRISPR-GO system can efficiently control the spatial positioning of genomic loci relative to specific nuclear compartments in the nucleus, including the nuclear periphery, Cajal bodies, promyelocytic leukemia (PML) bodies and heterochromatin. Built on an intrinsic stability switch of CRISPR guide RNAs, CRISPR LiveFISH uses fluorescent oligonucleotides for DNA and RNA tracking in a broad range of cell types, including primary cells. LiveFISH tracks the real-time movement of DNA double-strand breaks induced by CRISPR-Cas9-mediated editing and consequent endogenous chromosome translocations. In addition, by combining Cas9 and Cas13 systems, LiveFISH allows for simultaneous visualization of genomic DNA and RNA transcripts in living cells. Thus, the CRISPR LiveFISH enables real-time live imaging of DNA and RNA dynamics during genome editing, transcription, and rearrangements in single cells.


联系人/Contact:

生命科学学院

于文越 yuwenyue@westlake.edu.cn