生命科学学院 理学院 工学院

Kiryl D. Piatkevich, Ph. D.

西湖大学提供世界一流的研究环境,让科学家有自由和机会释放他们的创造力,以解决社会最紧迫的问题。西湖大学还将提供全新的教学和指导范式,以培养下一代科学领袖。在这里,每位学生、每位教师都有机会来创造和塑造西湖大学的未来。

个人简介

Kiryl Piatkevich为西湖大学生命科学学院助理教授。从幼年开始,Piatkevich博士就对化学产生了极大的兴趣。出于对科学的热情,他15岁于白俄罗斯国立大学Lyceum开始学习化学专业。从Lyceum毕业后,Piatkevich博士继续在罗蒙诺索夫莫斯科国立大学攻读硕士学位,同时从事有机金属、分析化学、物理化学和生物有机化学等研究。在获得荣誉理学硕士学位后,Piatkevich博士成功获得继续攻读莫斯科国立大学与纽约阿尔伯特爱因斯坦医学院联合培养博士的机会,致力于开发小型哺乳动物活体成像的创新方法。获得化学博士学位后,Piatkevich博士作为博士后研究员加入麻省理工学院光遗传学和神经工程学先驱Edward Boyden博士领导的合成神经生物学实验室。在此期间,Piatkevich博士致力于利用合成生物学研发神经接口的新方法,并研发出了几种新的分子技术用于观察活体动物的神经元活动。Piatkevich博士是第一个在主要神经科学模型生物(包括蠕虫、鱼和小鼠)中以高时间精度进行神经元活动的光学记录的科学家。从2019年开始,Piatkevich博士在西湖大学生命科学学院独立领导分子生物工程实验室(实验室网址:www.piatkevich-lab.com)。

学术成果

Piatkevich博士的研究方向是研发和设计尖端分子和成像技术,用于分析、控制和修复复杂生物系统,如大脑。设计用于光学记录和操控神经元活动的遗传编码分子工具,以及用于神经元解剖学追踪的荧光探针。这是一个跨学科研究计划,涵盖合成和化学生物学、生物工程、生物化学、生物技术、分子体内成像、神经科学、机器人和细胞生物学等领域。Piatkevich实验室将系统地应用其研发的技术来揭示脑紊乱的分子机制并揭示神经编码的基本原理。新的分子技术也会用于通过光创建先进的脑机接口。

代表论文

1. Piatkevich K.D.#, Bensussen S.#, Tseng H.#, Shroff S.N., Lopez-Huerta V.G., Park D., Jung E.E., Shemesh O.A., Straub C., Gritton H.J., Romano M.F, Costa E., Sabatini B.L., Fu Z, Boyden E.S., Han X. Population imaging of neural activity in awake behaving mice. Nature 2019, in press (# equal contribution).

2. Qian Y.#, Piatkevich K.D.#, McLarney B.#, Abdelfattah A.S., Mehta S., Gottschalk S., Molina R.S., Zhang W., Drobizhev M., Hughes T.E., Zhang J., Schreiter E.R., Shoham S., Razansky D., Boyden E.S., Campbell R.E. A Genetically Encoded Near-Infrared Fluorescent Calcium Ion Indicator. Nature Methods, 16 (2), 171 (# equal contribution).

3. Piatkevich K.D.#, Jung E.E.#, Straub C., Linghu C., Park D., Suk H.J., Hochbaum D.R., Goodwin D., Pnevmatikakis E., Pak N., Kawashima T., Yang C.-T., Rhoades J.L., Shemesh O., Asano S., Yoon Y.-G., Freifeld L., Saulnier J., Riegler C., Engert F., Hughes T., Drobizhev M., Szabo B., Ahrens M.B., Flavell S.W., Sabatini B.L., Boyden E.S. A Robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nature Chemical Biology, 14 (4), 352 (# equal contribution).

4. Piatkevich K.D., Suk H.J., Suhasa B.K., Yoshida F., DeGennaro E.M., Dro bizhev M., Hughes T.E., Desimone R., Boyden E.S.*, Verkhusha V.V. Near-infrared fluorescence proteins engineered from bacterial phytochromes in neuroimaging. Biophys. J. 113(10), 2299-2309.

5. Piatkevich K.D., English B.P., Malashkevich V.N., Xiao H., Almo S.C., Singer R.H., Verkhusha V.V. Photoswitchable red fluorescent protein with a large Stokes shift. Chem Biol. 2014. 21:1402.

6. Piatkevich K.D.*, Murdock M.H., Subach F.V.* Advances in engineering and application of optogenetic indicators for neuroscience. Applied Sciences. 2019, 9(3), 562. (*co-corresponding).

7. Tillberg P.W.#, Chen F.#, Piatkevich K.D., Zhao Y., Yu C.C., English B.P., Gao L., Martorell A., Suk H.J., Yoshida F., DeGennaro E.M., Roossien D.H., Gong G., Seneviratne U., Tannenbaum S.R., Desimone R., Cai D., Boyden E.S. Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nature Biotechnology. 34 (9), 987-992 (# equal contribution).

8. Shemesh O.A., Tanese D., Zampini V., Linghu C., Piatkevich K., Ronzitti E., Papagiakoumou E., Boyden E.S., Emiliani V. Temporally precise single-cell resolution optogenetics. Nature Neuroscience. 20(12), 1796-1806.

9. Subach O.M, Barykina N.V., Anokhin K.V., Piatkevich K.D., Subach F.V. Near-Infrared Genetically Encoded Positive Calcium Indicator Based on GAF-FP Bacterial Phytochrome. Int. J. Mol. Sci., 20(14), 3488.

10. Subach O.M., Kunitsyna T.A., Mineyeva O.A., Lazutkin A.A., Bezryadnov D.V., Barykina N.V., Piatkevich K.D., Ermakova Y.G., Bilan D.S., Belousov V.V., Anokhin K.V., Enikolopov G.N.*, Subach F.V.* Slowly reducible genetically encoded green fluorescent indicator for in vivo and ex vivo visualization of hydrogen peroxide. Int. J. Mol. Sci. 20(13), 3138;

11. Barykina N.V., Doronin D.A., Subach O.M., Sotskov V.P., Plusnin V.V., Ivleva O.A., Gruzdeva A.M., Kunitsyna T.A., Ivashkina O.I., Lazutkin A.A., Malyshev A.Y., Smirnov I.V., Varizhuk A.M., Pozmogova G.E., Piatkevich K.D., Anokhin K.V., Enikolopov G.N., Subach F.V*. NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics. Scientific Reports, 8 (1), 15233.

12. Doronin D., Barykina N., Subach O., Isaakova E., Varizhuk A., Pozmogova G., Piatkevich K., Anokhin K., Enikolopov G., Subach F.* Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast. BMC Biotechnology, 18 (1), 10.

13. Barykina N.V., Subach O.M., Piatkevich K.D., Jung E.E., Malyshev A.Y., Smirnov I.V., Bogorodskiy A.O., Borshchevskiy V.I., Varizhuk A.M., Pozmogova G.E., Boyden E.S., Anokhin K.V., Enikolopov G.N., Subach F.V.*. Green fluorescent genetically encoded calcium indicator based on calmodulin/M13-peptide from fungi. PLoS One. 12(8):e0183757.

14. Rowlands C.J., Park D., Bruns O.T., Piatkevich K.D., Fukumura D., Jain R.K., Bawendi M.G., Boyden E.S., So P.T.C.*. Wide-field three-photon excitation in biological samples. Light: Science & Applications. 6, e16255.

15. Barykina N.V., Subach O.M., Doronin D.A., Sotskov V.P., Roshchina M.A., Kunitsyna T.A., Malyshev A.Y., Smirnov I.V., Azieva A.M., Sokolov I.S., Piatkevich K.D., Burtsev M.S., Varizhuk A.M., Pozmogova G.E., Anokhin K.V., Subach F.V.*, Enikolopov G.N.*. A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites. Scientific Reports. 6:34447.

16. Konold P.E., Yoon E., Lee J., Allen S.L., Chapagain P.P., Gerstman B.S., Regmi C.K., Piatkevich K.D., Verkhusha V.V., Joo T., Jimenez R.*. Fluorescence from multiple chromophore hydrogen-bonding states in the far-red protein TagRFP675. J. Phys. Chem. Lett. 7, 3046-3051.

联系方式

电子邮箱:kiryl.piatkevich@westlake.edu.cn